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Advances in Neurobiology

Dent Lab

Supplemental movies from our most recent study in Journal of Neuroscience (in press):

Activity-Dependent Dynamic Microtubule Invasion of Dendritic Spines

 

Xindao Hu, Chris Viesselmann, Sookin Nam, Elliott Merriam and Erik W. Dent

COMING SOON...

Figure 1C:  A phase contrast movie of a newly plated control cortical neuron on a poly-D-lysine substrate.  Note the continued extension and retraction of filopodia.  Time = 10 minutes, images captured every 5 seconds.

Dent et al., 2007  Nature Cell Biology

Figure 1D:  A phase contrast movie a newly plated Ena/VASP-null (mmvvee) cortical neuron on a poly-D-lysine substrate.  Note the lack of membrane protrusion but continued retrograde flow throughout the lamellipodium.  Time = 10 minutes, images captured every 5 seconds.

Figure 1E:  A phase contrast movie of a control neuron forming a neurite.  Note that there are many filopodia in the region where the neurite forms with no one dominating to give rise to the neurite.  Rather, over a period of several hours filopodial and veil protrusions give rise to a growth cone that consolidates into a neurite.

Figure 1F:  A phase contrast movie of an mmvvee neuron forming a neurite.  In contrast to the control neuron in movie 7 the mmvvee neuron is able to extend a filopodium that is stabilized over a period of several hours.  This filopodium dilates to form a neurite that continues to extend, forming an axon over a period of several days.

Figure 1G:  Another example of an mmvvee neuron forming a neurite through extension, stabilization and dilation of a single filopodium.

Figure 5:  A movie of a control and mmvvee neuron transfected with mCherry-b-actin and EGFP-a-tubulin. Only the control cell has extensive actin bundles that give rise to filopodia. Note the extensive microtubule invasion into the periphery of each neuron.  However, in the mmvvee neuron microtubules turn parallel to the membrane and are brought back in retrograde flow.  Time = 10 minutes, images captured every 5 seconds.

Figure 7C:  A phase contrast movie of a newly plated mmvvee neuron on a poly-D-lysine and laminin substrate.  Note the extensive dynamic filopodia along the segmented periphery of the cell.  Time = 10 minutes, images captured every 5 seconds.

Figure 7E:  A phase contrast movie of an mmvvee neuron plated on a poly-D-lysine and laminin substrate.  Note the extensive filopodia and segmented regions around the periphery of the cell that give rise to neurites and subsequently a single axon.  Time = 25 hours, images captured every 2 minutes.

Figure 8E:  A movie of an mmvvee neuron transfected with mCherry-b-actin and EGFP-a-tubulin (not shown) and treated with blebbistatin (time of blebbistatin addition indicated on upper right of movie).  Note that within 30 minutes a number of actin-rich filopodia emerge from an otherwise lamellar periphery.  Time = 1:26 hrs, images captured every 20 seconds.